A method was established for the separation of complex and low content components in Houttuynia cordata Thunb by column-switching technology employing two reverse phase liquid chromatographic columns.A Synergi Fusion RP column and an Acclaim-120 C18 column with 250 mm×4.6 mm i.d.were adopted as the precolumn and analytical column，respectively.The precolumn was eluted with methanol -0.05% phosphoric acid as mobile phase.The analytical column was eluted with 0.05% phosphoric acid-acetonitrile.Three flavonoid components in Houttuynia cordata Thunb were simultaneously separated and detected.The calibration curves were linear in the concentration range of 10.0-1 000.0 mg?L-1，with correlation coefficient not less than 0.998.The recoveries of three compounds in Houttuynia cordata Thunb ranged from 95% to 99% with RSDs less than 3%.The results showed that the developed method was simple and rapid，and could be used in the analysis of flavonoid components.