A methodology regarding the preparation and certification of certified reference material (CRM) of allure red solution was presented.After the qualitative analysis on pure substance of allure red by liquid chromatography-mass spectrometry(LC-LTQ/MS) and 1H NMR spectrum,the purity of allure red was certified by high performance liquid chromatography(HPLC),the purity certification of allure red was performed at 240 nm with methanol and 0.1 mol/L ammonium acetate solution as mobile phase,and the Intersil ODS-C18 column(250 mm×4.6 mm,5 μm) was used for analysis.The certification purity of allure red was 99.61%.To verify the result，six certification labs were performed,at the same time,the water content and volatile impurity were determined by the total gravimetric analysis(TGA)．Allure red solution CRM was prepared by the gravity-volume method．After the tests of homogeneity and stability，the concentration of allure red solution CRM was certified and the uncertainty was evaluated．The certified result was 100 mg/L and the expanded relative uncertainty was 1.0%(k=2).The allure red solution CRM was approved as a national CRM and could be applied in food safety detection.