Determination of 14 Heterocyclic Aromatic Amines in Meat Products by Liquid Chromatography-Ion Trap-Time of Flight Tandem Mass Spectrometry
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Determination of 14 Heterocyclic Aromatic Amines in Meat Products by Liquid Chromatography-Ion Trap-Time of Flight Tandem Mass Spectrometry
Vol. 30, Issue 12, Pages: 1377-1381(2011)
作者机构:
1. 上海海洋大学食品学院
2. 上海出入境检验检疫局
3. 岛津国际贸易(上海)有限公司
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Determination of 14 Heterocyclic Aromatic Amines in Meat Products by Liquid Chromatography-Ion Trap-Time of Flight Tandem Mass Spectrometry. [J]. 30(12):1377-1381(2011)
DOI:
Determination of 14 Heterocyclic Aromatic Amines in Meat Products by Liquid Chromatography-Ion Trap-Time of Flight Tandem Mass Spectrometry. [J]. 30(12):1377-1381(2011)DOI:
Determination of 14 Heterocyclic Aromatic Amines in Meat Products by Liquid Chromatography-Ion Trap-Time of Flight Tandem Mass Spectrometry
A rapid analytical method was developed for the determination of 14 heterocyclic aromatic amines(HAAs) in meat products by liquid chromatography-ion trap-time of flight tandem mass spectrometry(LCMS-IT-TOF).The samples were extracted with acetonitrile.The extract was cleaned up with Phenomenex strata-X-C,eluted with methanol-ammonia(9∶1),then evaporated with a stream of nitrogen to near dryness and redissoved with acetonitrile-water(1∶9).The separation of HAAs was performed on a Phenomenex Kinetex 2.6μ C18 100A (2.1 mm i.d.×100 mm,2.6 μm),using 30 mmol/L ammonium formate-acetonitrile as mobile phase by gradient elution.The analytes were detected under positive ion electrospray ionization mode.Results showed that the linear ranges of 14 HAAs were 5-250 μg/L with limits of detection(S/N=3) of 0.59-3.27 μg/kg.The mean recoveries of all the compounds at three spiked levels of 10,50,250 μg/kg were in the range of 69%-107%,67%-106% and 76%-113%,respectively,with the corresponding RSDs(n=6) of 1.5%-15.6%,1.3%-11.8% and 1.8%-9.7%,respectively.The established method showed a wide liner range and a high sensitivity,and could meet the requirements for the simultanous analysis of the HAAs in meat products.
关键词
液相色谱-离子阱-飞行时间串联质谱(LCMS-IT-TOF)肉制品杂环胺(HAA)
Keywords
liquid chromatography-ion trap-time of flight tandem mass spectrometry(LCMS-IT-TOF)meat productsheterocyclic aromatic amines(HAAs)