A method of microwave assistant extraction（MAE） combined with high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）was developed for the determination of 10 active components in Flos Lonicerae.The Flos Lonicerae samples were extracted by MAE,using ethanol-H2O（7∶3） as solvent with a solid-liquid ratio of 1∶30 at a temperature of 70 ℃.The extracts were separated on an Agilent Poroshell 120 SB-C18（100 mm × 2.1 mm,2.7 μm） column by gradient elution of 0.5% formic acid-acetonitrile.A triple quadrupole MS operating in multiple-reaction monitoring（MRM） mode with negative ion was used for the quantitative analysis of 10 active components.Under the optimized conditions,10 constituents in Flos Lonicerae were analyzed completely within 10 min.The linear ranges of 10 active components were in the range of 0.05-500 mg/L,with correlation coefficients no less than 0.996 9.The limits of detection and limits of quantitation were in the ranges of 69-4 413 μg/kg and 231-14 709 μg/kg,respectively.The spiked recoveries ranged from 94% to 105%.The method was applied in the determination of 10 active components in six different Flos Lonicerae samples with contents of 3.98-14 356.31 mg/kg.The proposed method was fast,accurate,and was suitable for the quality control of Flos Lonicerae.