A chiral capillary column with gas chromatographic method was developed for the determination of three isomers of 2,3-butanediol,e.g.（2S,3S）-（+）-butanediol（d-BT for short），（2R,3R）-（-）-butanediol（l-BT for short）and meso-（R,S）-butanediol（meso-BT for short）,in liquor sample.The samples were extracted via vibration with ethyl acetate and methanol（4∶1） mixed solution.The extract was purified with graphitization carbon black column using solid phase extraction device.The 2,3-butanediol isomers were separated on a CP-Chirasil-DEX CB capillary column,and detected with flame ionization detector.The quantification of three isomers was performed by the external standard method.The calibration curves of d-BT,l-BT and meso-BT were linear in the ranges of 10-75,60-300,20-100 mg/L,respectively,with correlation coefficients of 0.999 1，0.999 6 and 0.999 9,respectively.The spiked recoveries of three butanediol isomers were 90%-113% in wine with RSDs（n=6）of 1.1%-7.2%,and 96%-99% in spirit with RSDs（n=6）of 1.7%-3.8%,respectively.The detection limit was 1.0 mg/L.The method was applied in the determinations of d-BT,l-BT and meso-BT in wine samples and sipirit samples before and after irradiated by Cobalt 60-gamma rays with irradiation dose of 10 kGy.The results showed that d-BT was detected in spirit samples after irradiation,but no obvious change of d-BT content occured in red wine,which could provide an evidence on whether the sipirit samples were irradiated or not.