A novel flow-injection chemiluminescence method was developed for the determination of paracetamol,which was based on the chemiluminescence reaction between paracetamol and hydroxy free radical produced by Fe3+ and H2O2 solution in the presence of dichlorofluorescein as energy transfer in the chemiluminescence system．Various factors influencing the chemiluminescence system were investigated and the possible mechanism was also discussed.Under the optimum conditions,the linear range for the determination of paracetamol was 8.0×10-8-5.0×10-5 mol/L with a detection limit（S/N=3） of 5.0×10-9 mol/L,the relative standard deviation（RSD,n=9） was 2.2% for 3.5×10-6 mol/L paracetamol solution.The method was successfully applied in the determination of paracetamol in pharmaceutical sample.