A liquid chromatographic method was established for the determination of 20 disperse dyes in drink and wine.The chromatographic conditions，including analysis column types，composition of mobile phase，detection wavelengths， were optimized.The sample was extracted with dichloromethane-ethyl ether mixed solution，and the separation of 20 dyes was performed on a C18 column，by using solution of 10 mmol/L ammonium acetate as mobile phase，at detection wavelengths of 430，570,640 nm.Under the optimal conditions，the calibration curves were liner in the range of 0.5-5.0 mg/L with correlation coefficients higher than 0.999.The recoveries of disperse dyes from drink and wine at spiked levels of 0.1,0.2,0.4 mg/kg were in the range of 80%-99% with RSDs of 0.1-9.1%.The result showed that the method was simple，rapid and accurate，and was suitable for the determination of disperse dyes in drink and wine.