A reversed-phase high performance liquid chromatographic method was developed for the determination of three alkaloids of Lycoris radiate.Chromatographic separation was performed on a ZORBAX ODS-C18 reversed phase column（150 mm × 4.6 mm，5 μm）.Effects of mobile phase,pH and flow rate on the analysis of alkaloids of Lycoris radiate were investigated.The optimized chromatographic conditions for the alkaloids of Lycoris radiate were as follows:mobile phase:09% triethylamine aqueous solution （pH 8.0 adjusted with acetic acid）（A）-acetonitrile（B）-methanol（C）,flow rate:1.0 mL /min,detection wavelength:234 nm.Under the optimal conditions,14 alkaloids were successfully separated from Lycoris radiate sample.The detection limits were 3.375 mg/L for galanthamine,0.475 mg/L for lycoramine and 0.495 mg/L for lycorine,respectively.The average recoveries were all 98%.The method was applied in the detection of three alkaloids contents in different parts of Lycoris radiate.The result indicated that the contents were significantly different in different parts.The highest content part of alkaloids was the leaves of Lycoris radiate.In the same part,the content of lycorine was the highest among them three,and that of galanthamine was the lowest.