A high performance liquid chromatography tandem electrospray ionization mass spectrometry（HPLC-ESI MS/MS） method was developed and validated for the determination of phenolic endocrine disrupting chemicals（EDCs），including bisphenol A（BPA）,triclosan（TCS）,17α-ethynylestradiol（EE2）,estrone（E1） and 17 β-estradiol（E2）in human urine.The samples were purified by liquid-liquid extraction,derivertized with dansyl chloride,and detected by HPLC-ESI MS/MS.The target compounds were quantified by the stable isotope dilution technique.The calibration curve were linear in the range of 0.2-100 μg/L for the 5 EDCs with correlation coefficients more than 0.99.The limits of detection of 5 EDCs were in the range of 0.02-0.27 μg/L.The matrix recoveries of the method for 5 EDCs at three spiked levels of 5,10 and 50 μg/L ranged from 85% to 125%.The relative standard deviations（RSDs,n=3） were between 0.53% and 14.4%.The method was successfully applied in the analysis of 5 EDCs in human urine with its sensitivity and accurancy .