A high performance liquid chromatographic(HPLC) method was developed for the determination of vitamin K1 in cosmetics.Different cosmetic samples were dispersed and extracted by adding saturated sodium chloride aqueous solution and hexane in appropriate sequence,and detected by HPLC with diode array detector(HPLC/DAD).The samples containing high level of waxes were further purified by silica-gel solid phase extraction.The extraction and purification procedures were optimized.Under the optimized conditions,the average recoveries of vitamin K1 were in the range of 99%-105% with relative standard deviations less than 5%.The limit of quantitation was 0.4 mg/kg.The method was accurate,rapid and widely applicable,and could be used to quantitatively analyze vitamin K1 in cosmetics.