A high performance capillary electrophoresis method for the simultaneous separatinon and determination of four anticoccidial drugs(e.g.sulfanilamide，sulfaclozine，sulfaquinoxaline，diclazuril) in soil was developed by using 0.1 mol/L NaOH and THF(3∶2) as extraction solution.The effects of some important factors such as pH value，composition and concentration of running buffer，separation voltage and separation temperature，were investigated,and the orthogonal test factors were optimized.The optimal conditions were as follows：the running buffer：50 mmol/L borax-200 mmol/L boric acid(pH 9.0),separation voltage：23 kV,separation temperature：25 ℃,UV detection wavelength：220 nm and 254 nm.Under the optimal chromatographic conditions，the four anticoccidial drugs can be well separated within 7.0 min.The calibration curves of four anticoccidial drugs were linear in the range of 1.56-50 mg/L with correlation coefficients(r2) of 0.994 7-0.999 9.The average recoveries of four anticoccidial drugs in soil sample at three spiked concentration levels of 2.0，5.0，10 mg/kg ranged from 77% to 90% ,with relative standard deviations(RSDs,n=6) of 6.3%-8.1%.The method was simple，rapid and low-cost,and was successfully applied in the determination of four anticoccidial drugs in the soil of poultry farms.