A simple and rapid method was developed for the simultaneous determination of 290 pesticide residues in tea.The sample was prepared by the modified QuEChERS(quick,easy,cheap,effective,rugged and safe) method,extracted with acetonitrile,followed by liquid-liquid partitioning formed by adding sodium chloride and anhydrous magnesium sulfate.The extract was separated on a Thermo Accucore aQ(100 mm×2.1 mm,2.6 μm) column,detected by high performance liquid chromatography-tandem mass spectrometry under multiple reaction monitoring mode and quantified by the external standard method.The calibration curves of 290 pesticide residues were linear in the range of 1.0-200 μg?L-1 with correlation coefficients more than 0.99.The recoveries for all compounds in tea at the fortified levels of 1MRL,2MRL and 4MRL were 67%-119%.The limits of quantitation(LOQs，S/N≥10) for 290 pesticides were less than 10.0 μg?kg-1.The established method was successfully used for the screening and determination of real samples(such as green tea,black tea,oolong tea and pu’er tea) bought at supermarket.The method was simple,rapid and reliable,and could meet the requirement for simultaneous determination of 290 pesticide residues in tea.