Molecularly imprinted polymers（MIPs) were synthesized by bulk polymerization using polydatin（POL) as template molecule,acrylamide（AM),4-vinylpyridine(4-VP),2-hydroxyethylmetacrylate(HEMA) or methacrylic acid（MAA) as functional monomer,and ethyleneglycol dimethacrylate（EGDMA) as crosslinking　agent,initiating thermally with azobisisobutyronitrile（AIBN).The binding and selectivity of MIPs to POL and different substrates were evaluated by the static batch method.MIP1,which was synthesized with AM as functional monomer,had the best recoginition property to the template,followed by MIP2 sythesized with 4-VP as functional monomer,MIP3 synthesized with HEMA as functional monomer and MIP4 synthesized with MAA as functional monomer.The results indciated that the interactions between the template and the functional monomer in the solvent influenced the recognition property of MIPs．Static equilibrium binding experiments and the scatchard analysis showed that MIP1 existed good selectivity and recognition properties to the template POL.Two kinds of binding sites were produced in the MIP1 matrix and their dissociation constants were calculated to be 7.43×10-5 mol/L and 3.70×10-3 mol/L,respectively.Compared with non-imprinted polymer(NIP1),the imprinted polymer(MIP1) exhibits a higher affinity for POL among the mixed substrates.The MIP1 was applied in the specific extraction of POL from Polygonum cuspidatum Sieb.et Zucc.extracts with satisfactory results.