A rapid method for the determination of glyceryltrioleate was developed by using screen-printed electrode（SPE） modified with lipase as an electrochemical biosensor.Cyclic voltammetry(CV) and chronocoulometry(CC) were applied to characterize the behavior of glyceryltrioleate on the surface of the lipase-modified SPE.The optimal conditions of glyceryltrioleate determination were investigated.A linear response to glyceryltrioleate in the concentration ranges of 001-5 g?L-1 with a detection limit of 2.5 mg?L-1 for CV,and 0.001-5.50 g?L-1 with a detection limit of 0.2 mg?L-1 for CC were obtained.The method was used in the determination of 6 sunflower seed oil samples with spiked recoveries of 94%-105%.