The application of a pretreatment instrument,SPD-600,in dioxin-like substances determination by a new enzyme-linked immunosorbent assay method was introduced in this paper,Its clean-up efficiencies and recoveries under different conditions were evaluated.The results indicated that SPD-600 achieved the optimum performance under a heated condition of 60 ℃ when almost all impurities were removed.And the recoveries of the labeled internal standards are comparable with that of the multilayer silica column treatment.In addition,an ELISA-based kinetic exclusion assay method using an automatic analysis instrument(DXS-600) was developed for dioxin analysis,which had avoided the competitive step.The results showed that DXS-600 eliminated the artificial effects and was more sensitive and stable than the traditional ELISA.Finally,21 flue gas samples were determined using SPD-600 combined with DXS-600 systems,and the results obtained were compared with those of the HRGC-HRMS analysis.A good correlation with r2 of 0.985 8 was found between two methods.Therefore,the method of SPD-600 clean-up combined with DXS-600 analysis can be used as a quick screening method for the determination of dioxin.