Determination of Cefotaxime Residue in Milk by Molecular Imprinted Solid Phase Extraction/Capillary Electrophoresis

您当前的位置：

首页 >

文章列表页 >

Determination of Cefotaxime Residue in Milk by Molecular Imprinted Solid Phase Extraction/Capillary Electrophoresis

- Determination of Cefotaxime Residue in Milk by Molecular Imprinted Solid Phase Extraction/Capillary Electrophoresis
- Vol. 31, Issue 10, Pages: 1334-1338(2012)
- 作者机构：
  
  1. 安徽农业大学生物技术中心
  2. 安徽农业大学动物科技学院
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
Determination of Cefotaxime Residue in Milk by Molecular Imprinted Solid Phase Extraction/Capillary Electrophoresis. [J]. 31(10):1334-1338(2012)
DOI：

Determination of Cefotaxime Residue in Milk by Molecular Imprinted Solid Phase Extraction/Capillary Electrophoresis. [J]. 31(10):1334-1338(2012) DOI：

摘要

采用分子印迹技术，以头孢噻肟（CTX）为模板分子，α-甲基丙烯酸（MAA）为功能单体，乙二醇二甲基丙烯酸酯（EGDMA）为交联剂合成了头孢噻肟分子印迹聚合物（CTX-MIP）。以该分子印迹聚合物为固相萃取柱填料，毛细管电泳进行检测，建立了分子印迹固相萃取/毛细管电泳检测牛奶中头孢噻肟残留的方法。结果表明，CTX-MIP对CTX具有较高的选择性，萃取效果良好。该方法在CTX为5～100 mg/L范围内呈良好线性，相关系数(r2)为0.999 4；3种不同加标水平（10、50、100 mg/L）的回收率分别为78%、84%、86%,相对标准偏差（RSD）为3.5%～4.5%,检出限（LOD，S/N≥3）为98.52 μg/L,定量下限（LOQ，S/N≥10）为329.0 μg/L，符合兽药残留分析的要求。

Abstract

A molecularly imprinted polymer for cefotaxime（CTX-MIP） was synthesized by the molecular imprinting technique using cefotaxime（CTX） as template molecule,α-methacrylic acid（MAA） as functional monomer,ethylene glycol dimethacrylate（EGDMA） as cross linking agent.By using the cefotaxime molecularly imprinted polymer as sorbent in solid-phase extraction column,a molecularly imprinted solid phase extraction-capillary electrophoresis（MISPE-HPCE） method was developed for the determination of cefotaxime residue in milk.The results showed that cefotaxime imprinted polymer exhibited a higher selectivity to cefotaxime compared with that of the non imprinted polymer.The linear range of cefotaxime was in the range of 0.5-100 mg/L，and the correlation coefficient was 0.999 4.The recoveries at three spiked concentration levels of 10,50,100 mg/L were 78%,84% and 86%,respectively,with RSDs of 3.5%-4.5%.The limit of detection(LOD,S/N≥3） and the limit of quantitation(LOQ,S/N≥10） were 98.52 μg/L and 329.0 μg/L,respectively.The method could meet the requirements for veterinary drug residue analysis.

关键词

分子印迹固相萃取毛细管电泳头孢噻肟牛奶

Keywords

molecularly imprinted solid phase extractioncapillary electrophoresiscefotaximemilk

references

Views

1237

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Rapid Screening of 69 Veterinary Drug Residues in Milk by Ultra-high Performance Liquid Chromatography-Quadrupole-Time-of-flight Mass Spectrometry

Determination of 51 Hormones in Milk by Ultrahigh Performance Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometry

Determination of Fluoroquinolones in Environmental Water Samples by Capillary Electrophoresis with Dispersive Liquid－Liquid Microextraction Based on Ultrasonic-assisted in-situ Formation and Solidification of Floating Deep Eutectic Solvent

Related Author

No data

Related Institution

College of Veterinary Medicine，China Agricultural University

School of Public Health，Capital Medical University

Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning，Beijing Center for Disease Prevention and Control

School of Environmental and Pharmaceutical Engineering，Taizhou Institute of Science and Technology，Nanjing University of Science and Technology

Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰