Electrochemical Determination of Methotrexate at Gold Nanoparticles Modified Carbon Paste Electrode and Its Interaction with Herring Sperm DNA

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Electrochemical Determination of Methotrexate at Gold Nanoparticles Modified Carbon Paste Electrode and Its Interaction with Herring Sperm DNA

- Electrochemical Determination of Methotrexate at Gold Nanoparticles Modified Carbon Paste Electrode and Its Interaction with Herring Sperm DNA
- Vol. 31, Issue 11, Pages: 1426-1430(2012)
- 作者机构：
  
  1. 延安大学化学与化工学院
  2. 延安市药检所
- 作者简介：
- 基金信息：
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Electrochemical Determination of Methotrexate at Gold Nanoparticles Modified Carbon Paste Electrode and Its Interaction with Herring Sperm DNA. [J]. 31(11):1426-1430(2012)
DOI：

Electrochemical Determination of Methotrexate at Gold Nanoparticles Modified Carbon Paste Electrode and Its Interaction with Herring Sperm DNA. [J]. 31(11):1426-1430(2012) DOI：

摘要

制备了纳米金修饰碳糊电极，使用循环伏安法(CV)研究了甲氨蝶呤(MTX)在该修饰电极上的电化学特性，并建立了纳米金修饰碳糊电极电化学测定MTX的新方法。利用线性扫描伏安法(LSV)和方波伏安法(SWV)研究了MTX与鲱鱼精DNA的相互作用。实验发现，在pH 3.6 HAc-NaAc缓冲溶液中，MTX在0.86 V处有一灵敏的氧化峰，氧化峰电流Ipa与MTX的浓度在0.5 ～10.0 μmol?L-1范围内呈良好的线性关系，方法检出限(S/N=3)为0.2 μmol?L-1。对3.0 μmol?L-1的MTX进行11次平行测定，其RSD为4.7%。该修饰电极可用于MTX样品的测定，结果满意。当不同浓度鲱鱼精DNA加入MTX溶液后，氧化峰电位正移，氧化峰电流降低，表明MTX与鲱鱼精DNA之间发生了相互作用，形成了非电活性化合物。电化学研究表明，MTX与鲱鱼精DNA之间的结合比为2∶1，结合常数为4.6×105 L?mol-1。

Abstract

A gold nanoparticles modified carbon paste electrode(GNPs/CPE) was prepared,and the electrochemical characteristics of methotrexate(MTX) at GNPs/CPE were studied by cyclic voltammetric method.A novel electrochemical method for the determination of MTX at GNPs/CPE was established and its interaction with herring sperm DNA(hsDNA) was investigated by linear sweep voltammetry(LSV) and square wave voltammetry(SWV).The results indicated that there was a good linear relationship between anodic peak current and MTX concentration in the range of 0.5-10.0 μmol?L-1,and the detection limit(S/N=3) was 0.2 μmol?L-1.The relative standard derivations(n=11) was 4.7% for 3.0 μmol?L-1 MTX.The method was applied in the determination of MTX in the injection samples with satisfactory results.The results also showed that,in the presence of various concentrations of hsDNA,the redox peak potential of methotrexate shifted positively and its peak current was decreased,which indicated that MTX could react with hsDNA and form an electrochemical inactive compound.The binding equilibrium constant and binding ratio were calculated to be 4.6×105 L?μmol-1 and 2∶1,respectively.

关键词

甲氨蝶呤纳米金修饰电极鲱鱼精DNA相互作用

Keywords

methotrexate(MTX)gold nanoparticles modified carbon paste electrodeherring sperm DNA(hsDNA)interaction

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Key Laboratory of Luminescence and Real-time Analysis of the Ministry of Education，School of Chemistry and Chemical Engineering，Southwest University

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