Online Multidimensional Separation System Based on the Biphasic Trap Column for Large Scale Phosphorylation Analysis of Human Liver
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Online Multidimensional Separation System Based on the Biphasic Trap Column for Large Scale Phosphorylation Analysis of Human Liver
Vol. 31, Issue 9, Pages: 1070-1074(2012)
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中国科学院大连化学物理研究所中国科学院分离分析化学重点实验室国家色谱研究中心
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Online Multidimensional Separation System Based on the Biphasic Trap Column for Large Scale Phosphorylation Analysis of Human Liver. [J]. 31(9):1070-1074(2012)
DOI:
Online Multidimensional Separation System Based on the Biphasic Trap Column for Large Scale Phosphorylation Analysis of Human Liver. [J]. 31(9):1070-1074(2012)DOI:
Online Multidimensional Separation System Based on the Biphasic Trap Column for Large Scale Phosphorylation Analysis of Human Liver
A fully automated online multidimensional separation system,which is based on a reversed phase-strong cation exchange(RP-SCX) biphasic trap column,was developed for the large scale phosphorylation analysis of human liver.Compared with the conventional online SCX-RP system and off-line mode,the biphasic column can reduce the sample loss and improve the detection sensitivity.This system was applied in the large scale phosphorylation analysis of human liver and a total of 3 082 unique phosphopeptides,including 3 056 unique phosphorylation sites corresponding to 1 332 phosphoproteins were identified under the FDR<1%.The molecular function and motif analysis of phosphoproteins has revealed the important role of phosphorylation in the liver physiological functions.
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