Simultaneous Determination of Thiophanate-methyl,2-Aminobenzimidazole,Carbendazim,Triabendazole and 5-Hydroxy-triabendazole in Concentrated Fruit Juices by LC-MS/MS
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Simultaneous Determination of Thiophanate-methyl,2-Aminobenzimidazole,Carbendazim,Triabendazole and 5-Hydroxy-triabendazole in Concentrated Fruit Juices by LC-MS/MS
Vol. 32, Issue 1, Pages: 84-88(2013)
作者机构:
1. 广西出入境检验检疫局检验检疫技术中心
2. 广西大学轻工与食品工程学院
3. 上海出入境检验检疫局动植物与食品检验检疫技术中心
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Simultaneous Determination of Thiophanate-methyl,2-Aminobenzimidazole,Carbendazim,Triabendazole and 5-Hydroxy-triabendazole in Concentrated Fruit Juices by LC-MS/MS. [J]. 32(1):84-88(2013)
DOI:
Simultaneous Determination of Thiophanate-methyl,2-Aminobenzimidazole,Carbendazim,Triabendazole and 5-Hydroxy-triabendazole in Concentrated Fruit Juices by LC-MS/MS. [J]. 32(1):84-88(2013)DOI:
Simultaneous Determination of Thiophanate-methyl,2-Aminobenzimidazole,Carbendazim,Triabendazole and 5-Hydroxy-triabendazole in Concentrated Fruit Juices by LC-MS/MS
A liquid chromatography tandem mass spectrometric method was developed for the determination of benzimidazole fungicides and their metabolites such as thiophanate-methyl(TM),2-aminobenzimidazole(2-AB),carbendazim(MBC),triabendazole(TBZ),5-hydroxy-triabendazole(5-OH-TBZ) in 7 types of concentrated fruit juices including apple,mango,pineapple,pear,orange,lychee and passion fruit.Seven representative concentrated fruit juices were selected and extracted with acetonitrile.The extracts were purified with PSA column.The separation of 5 analytes was carried out on a ZORBAX Eclipse XDB-C18 column using methanol-10 mmol/L ammonium acetate(containing 0.1% formic acid) as mobile phase by gradient elution.The detection of analytes was performed by electrospray tandem mass spectrometry with ES+ and multiple reaction monitoring (MRM) modes.The results showed that,the method showed good linear relationships in the range of 0.02 -0.2 mg/L for the tested samples,with correlation coefficients no less than 0.998 5.The quantitation limits were 2.0 μg/kg for 2-AB and 5-OH-TBZ,and 1.0 μg/kg for the rest of the analytes.The mean recoveries ranged from 70% to 110% with relative standard deviations of 0.1%-10.9% when the fortified levels for 2-AB and 5-OH-TBZ were 2,4,20 μg/kg and those for TBZ,MBC,TM were 1,2,10 μg/kg,respectively.The proposed method was sensitive and reliable,and was successfully applied in the determination of benzimidazole fungicides and their metabolites in real commercial concentrated juices.
关键词
LC-MS/MS浓缩果汁甲基硫菌灵2-氨基苯并咪唑多菌灵噻菌灵5-羟基噻菌灵
Keywords
liquid chromatography tandem mass spectrometryconcentrated fruit juicesthiophanate-methyl2-aminobenzimidazolecarbendazimtriabendazole5-hydroxy-triabendazole