1. 国家海洋局第一海洋研究所海洋生态研究中心
2. 青岛科技大学化学与分子工程学院
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Oil Fingerprint Analysis of Bicyclic Sesquiterpanes by Internal Standard Method and Oil Identification. [J]. 31(11):1421-1425(2012)
采用气相色谱-质谱联用(GC-MS)技术,建立了原油中双环倍半萜化合物内标法半定量的分析方法,确定了基于谱图特征、确认离子m/z 123、179、193、207及计算保留指数的组分定性方法,对10种双环倍半萜化合物进行了定性、定量分析。对不同地理位置海上油井平台9个原油的双环倍半萜指纹进行了分析。研究结果表明:原油中双环倍半萜化合物总含量在600~8 000 μg/g之间;各原油中均以4,4,8,9,9-五甲基十氢化萘(BS3)、4,4,8,9,9-五甲基十氢化萘(BS4)、C16-sesquiterpane(BS8) 、8β(H)-升补身烷(BS10)化合物的含量较高,占双环倍半萜总量的60%以上;不同地理位置的原油中双环倍半萜含量的分布规律为:涠洲岛海区>渤海海区>进口原油G1、G2;国外原油G3的双环倍半萜含量与渤海原油B3的含量相当,但分布形式不同。同一区块不同采油平台原油中的双环倍半萜指纹非常相似,不同来源的原油中双环倍半萜化合物的分布特征及含量有很大的差异。通过此方法可以实现不同来源原油的准确鉴别。
A semiquantitative gas chromatography-mass spectrometric method relative to internal standard was developed for the fingerprinting analysis of bicyclic sesquiterpanes in crude oils.Based on the chromatograms properties,characteristic fragment ions(m/z 123,179,193 and 207) and the calculated retention indices,10 bicyclic sesquiterpanes compounds were identified and the concentration formulas of semiquantitation using the internal standard(d18-decahydronaphthalene) were provided.Bicyclic sesquiterpanes fingerprinting of 9 crude oils collected from Weizhou island,Bohai sea and crude oil from import sources were analyzed.The result revealed the following situation that the total concentrations of bicyclic sesquiterpanes range from 600 μg/g to 8 000 μg/g.The dominating components in all the studied crude oils are C15-sesquiterpane(BS3),C15-sesquiterpane(BS4),C16sesquiterpane(BS8) and 8β(H)-homodrimane(BS10),which account for 60% of total contents of bicyclic sesquiterpanes.The abundances of bicyclic sesquiterpanes in different oils follow an increasing order as Weizhou island > Bohai sea > crude oil from imports G1,G2.The total bicyclic sesquiterpane contents of G3(crude oil of GIRASSOL) and B3(crude oils from Bohai sea) are similar but exhibit different distributions of bicyclic sesquiterpane signatures.Crude oils from different offshore oil platforms in the same block exhibit very similar bicyclic sesquiterpanes signatures;In contrast,oils from different sources have different signatures of both the concentrations and relative distribution patterns of bicyclic sesquiterpanes compounds.Fingerprinting analysis of bicyclic sesquiter-panes in crude oils by semiquantitative method can achieve the identification of different crude oils.
原油双环倍半萜指纹半定量法
crude oilbicyclic sesquiterpanes fingerprintsemiquantitative method
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