Preparation,Characterization of Titanium Dioxide Nanoparticles and Its Application in an Alpha-fetoprotein Immunosensor

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Preparation,Characterization of Titanium Dioxide Nanoparticles and Its Application in an Alpha-fetoprotein Immunosensor

- Preparation,Characterization of Titanium Dioxide Nanoparticles and Its Application in an Alpha-fetoprotein Immunosensor
- Vol. 32, Issue 11, Pages: 1322-1327(2013)
- 作者机构：
  
  内江师范学院化学化工学院
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Preparation,Characterization of Titanium Dioxide Nanoparticles and Its Application in an Alpha-fetoprotein Immunosensor. [J]. 32(11):1322-1327(2013)
DOI：

Preparation,Characterization of Titanium Dioxide Nanoparticles and Its Application in an Alpha-fetoprotein Immunosensor. [J]. 32(11):1322-1327(2013) DOI：

摘要

以猪皮胶原和硫酸钛为原料，经过鞣制反应高温煅烧后，制得纳米二氧化钛(TiO2)。基于静电吸附作用，将制备的二氧化钛(TiO2)与壳聚糖(Chitosan)纳米复合物固定在金电极上，利用壳聚糖上丰富的氨基吸附纳米金颗粒，最后吸附甲胎蛋白抗体，从而成功制得甲胎蛋白免疫传感器。通过紫外可见光谱(UV-Vis)、红外光谱(FT-IR)、X射线衍射(XRD)和透射电镜(TEM)对纳米二氧化钛粒子进行表征。在最优实验条件下，该免疫传感器对AFP的线性检测范围为0.01~100 ng/mL，相关系数为0.998 2，检出限为0.003 ng/mL。

Abstract

Using pig collagen and titanium sulfate as raw material，by tanning reaction with high temperature calcination，the nanometer titanium dioxide was prepared.Then，fixing the nanometer titanium dioxide-chitosan（Cs） nanocomposites on gold electrodes through electrostatic adsorption，using abundant amino in Cs to absorb gold nano particles and lastly absorb alpha-fetoprotein antibodies,an AFP immunosensor was successfully fabricated.The techniques of UV-vis spectroscopy(UV-Vis)，infrared spectroscopy(FT-IR)，X ray diffraction(XRD) and transmission electron microscopy were used to characterize the nanometer titanium dioxide.Under the optimal conditions，the immune sensor showed a good linear relationship for AFP in the range of 0.01-100 ng/mL，with a correlation coefficient of 0.998 2 and a detection limit of 0.003 ng/mL.

关键词

胶原模板纳米二氧化钛壳聚糖纳米金甲胎蛋白免疫传感器

Keywords

collagenstencilnano titanium dioxidechitosangold nanoparticlesα-fetoprotein(AFP)immunosensor

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湖南农业大学东方科技学院

湖南农业大学理学院应用化学系

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College of Chemistry Engineering，Yantai Nanshan University

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