An immunoaffinity column purification and high performance liquid chromatographic（IAC/HPLC） method was established for the simultaneous determination of 16 sulfonamide(SAs) residues in animal-originatal food products.The samples were extracted with 80% ethanol,cleaned-up with an immunoaffinity column,and analyzed by HPLC.The separation of 16 SAs was performed on an Agilent Eclipse XDB-C18 column using methanol(A)-pH 3.5 phosphate buffer(B) as mobile phase by gradient elution at a flow rate of 1.0 mL/min.The detection wavelength was set at 270 nm.The injection volume was 50 μL.All of 16 SAs residues were simultaneously separated and determined by HPLC within 42 min.Linear ranges of 16 SAs were in the range of 10-200 μg/L with correlation coefficients more than 0.999.The average recoveries at spiked concentration levels of 20,50 and 100 μg/kg ranged from 62% to 104%,with relative standard deviations(RSDs) of 0.9%-6.6%.The detection limits(S/N=3) ranged from 4 μg/kg to 10 μg/kg.The method was suitable for the determination of sulfonamide residues in the edible animal products,with advantages of simplicity，rapidness，sensitivity and environment friendliness.