A microchip capillary electrophoresis with contactless conductivity detection method was developed for the rapid determination of glucose and sodium chloride in the injection preparation.The pH value and constituent of running buffer were investigated to gain the effective resolution of the two ingredients.The excitation frequency of the detector was also surveyed in view of the signal response and detection sensitivity.The optimal conditions were as follows:separation media:20 mmmol/L borate（pH 9.6）,separation voltage:1 200 V,excitation frequency:70 kHz.Under the optimal conditions,the two analytes could be successfully separated within 60 s.Good linearities（r≥0.992） for NaCl and glucose were obtained in the ranges of 20-1 000 μmol/L and 50-5 000 μmol/L,respectively.The limits of detection（S/N=3∶1） were 8.2 μmol/L and 26.7 μmol/L,respectively.The spiked recoveries for Na+ and glucose were 95%-99% and 97%-104%,respectively,with relative standard deviations（RSDs,n=3） less than 4.0%.The present work provided an approach of choice to the simultaneous quantitative analysis of glucose and NaCl in the compound preparation widely used in clinical therapeutics.With the advantages of convenience,rapidity,considerable accuracy and stability,this method was applied in the determination of Na+ and glucose in real sample with satisfactory result.