A liquid chromatography-tandem mass spectrometric（LC-MS/MS） method with solid phase extraction was developed for the determination of 7 plant growth regulators（PGRs），including 2,4-dichlorophenoxyacetic acid，gibberellic acid，indole-3-butyric acid，α-naphthaleneacetic acid，4-fluorophenoxyacetic acid，4-chlorophenoxyacetic acid and indole-3-acetic acid，in orange.The samples were extracted with acetonitrile（contain 2% formic acid），and cleaned up on a Poly-Sery MAX.The extract was eluted with 4% formic acid in methanol，then evaporated with a stream of nitrogen to near dryness，and redissoved with acetonitrile-water（1∶9）. The targeted compounds were separated on a Phenomenex Gemini-NX 5u C18 110A（2.0 mm i.d.×150 mm，5.0 μm），and determined by LC-MS/MS under MRM mode with the matrix-matched external standard method.The results indicated that the calibration curves were linear in the certain concentration range with correlation coefficients greater than 0.99.The limits of detection（LODs，S/N>3） were in the range of 0.3-3.5 μg/kg and the limits of quantitation（LOQs，S/N>10） were 5.4-57.7 μg/kg.The recoveries of 7 PGRs in orange at three spiked levels ranged from 79.6% to 110.3% with relative standard deviations（RSDs） of 3.1%-10.0%.The established method was simple and sensitive，and could meet the requirements for simultanous analysis of PGRs in amphisarcas.