A novel electrochemical sensor for pyrazosulfuron-ethyl（PSE）detection based on molecularly imprinted polymer membrane was prepared by electropolymerization of o-aminophenol（oAP）at gold electrode in the presence of template molecule.The modification procedure was characterized via cyclic voltammetry and electrochemical impedance spectroscopy.And its recognition performance was evaluated by differential pulse voltammmograms（DPV）.Under the optimized conditions，the response current of K3Fe（CN）6 probe on the proposed sensor decreased linearly with PSE concentration in the range of 2-150 μmol/L with a detection limit of 0.65 μmol/L（r2=0.995）.With high selectivity and stability，the imprinted sensor was applied in the determination of PSE in environmental water samples with recoveries of 97.6%-104.4%.