Determination of Vitamin B2 Content by NMR

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Determination of Vitamin B2 Content by NMR

- Determination of Vitamin B2 Content by NMR
- Vol. 32, Issue 12, Pages: 1511-1514(2013)
- 作者机构：
  
  1. 华中科技大学同济医学院药学院
  2. 华南理工大学分析测试中心
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Determination of Vitamin B2 Content by NMR. [J]. 32(12):1511-1514(2013)
DOI：

Determination of Vitamin B2 Content by NMR. [J]. 32(12):1511-1514(2013) DOI：

摘要

建立了维生素B2片剂中维生素B2含量的核磁共振测定方法。核磁共振的测定条件：脉冲程序为cpmgpr1d，氢谱宽度为6 009 Hz，脉冲宽度为11.95 μs，延迟时间为6.50 μs，采样温度为27 ℃，采样次数为32次。以参比物质3-（三甲基硅基）氘代丙酸钠（TSP）为内标，将不同浓度标准品与内标的NMR峰面积比对维生素B2浓度绘制标准曲线，结果显示，维生素B2在 1.25~10 mg/mL范围内呈良好线性，其线性方程为：y=2.989 4 x+0.185 5，r2=0.999 2。方法的加标回收率为83.2%。该方法灵敏度高、重现性好、操作简便，可用于维生素B2片剂中维生素B2含量的测定。

Abstract

A NMR method was developed for the determination of vitamin B2（VB2） in vitamin B2 tablets.NMR determination conditions were as follows：the pulse sequence:cpmgpr1d，hydrogen spectral width:6 009 Hz，pulse width:11.95 μs，delay time:6.50 μs，temperature:27 ℃,number of scans:32.A good linear relationship was obtained between peak area ratio of VB2 to TSP and concentrations of VB2 in the range of 1.25-10 mg/mL.The regression equation was y=2.989 4 x+0.185 5（r2=0.999 2）.The average recovery was 83.2 %.The results indicated that the NMR based method had high sensitivity and good repeatability，and could be used in the determination of VB2 in vitamin B2 tablets.

关键词

核磁共振氢谱维生素B2含量测定

Keywords

NMRvitamin B2quantification

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青岛科技大学化学与分子工程学院

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