A rapid analytical method based on C18 solid phase extraction disk followed by high performance liquid chromatography(HPLC) for the determination of six trace sulfonylurea herbicides,including tribenuron,metsulfuron-methyl,chlorsulfuron,bensulfuron-methyl,ethametsulfuron,chlorimuron-ethyl in water was developed.Chromatographic conditions were optimized，and the factors that influence the extraction,including type and volume of desorption solvent,pH value of sample and amount of salt addition were investigated.Experimental results showed that,the extraction efficiencies for 6 sulfonylurea herbicides in water were the highest by C18 SPE disk adsorption when the water samples were adjusted to pH 3.0,and the target compounds adsorped on C18 were eluted with 10 mL of methanol.Under the optimal conditions,good linear relationships(r2≥0.999 0) were obtained between peak areas and concentrations of 6 sulfonylurea herbicides in the ranges of 0.10-20.0 μg/L for tribenuron and 0.05-20 μg/L for the rest of the target compounds.The detection limits were in the range of 0.019-0.037 μg/L.The recoveries of 6 sulfonylurea herbicides in spiked water sample and seawater sample were in the ranges of 92.6%-105.7% and 89.8%-108.7%,with relative standard deviations (RSDs) of 0.7%-7.1% and 0.5%-4.0%,respectively.The method was simple,rapid,accurate and sensitive,and could provide a new option for the determination of sulfonylurea herbicides in environmental water.