An accelerated solvent extraction（ASE）/gas chromatography-tandem mass spectrometric（ASE/GC-MS/MS） method combined with solid phase extraction（SPE） was developed for the simultaneous determination of 71 pesticides residues in marine sediments．The best extraction temperature and static time were 100 ℃ and 5 min in 1 cycle，respectively.Under the conditions，the sample was extracted with a mixture of hexane-acetone（1∶1）,cleaned up on a Carbon SPE cartridge and determined by MS/MS under multiple reaction monitoring mode（MRM）.The calibration curves showed good linearities（r2＞0.99） in the range of 1.0 -500 μg?L-1 for all pesticides，and the limits of detection（LOD,3σ） ranged from 0.29 μg?kg-1 to 108.43 μg?kg-1.The recoveries of the pesticides at a spiked level of 100 μg?kg-1 were in the range of 51.3%-104.6%with RSDs（n=6） of 0.1%-13.5%.