Flourescence Enhancement of CT2R by Silver Nanoparticles and Its Application in Determination of BSA

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Flourescence Enhancement of CT2R by Silver Nanoparticles and Its Application in Determination of BSA

- Flourescence Enhancement of CT2R by Silver Nanoparticles and Its Application in Determination of BSA
- Vol. 33, Issue 10, Pages: 1209-1212(2014)
- 作者机构：
  
  1. 湖南长沙环境保护职业技术学院
  2. 湖南科技大学化学化工学院
  3. 理论化学与分子模拟省部共建教育部重点实验室
- 作者简介：
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Flourescence Enhancement of CT2R by Silver Nanoparticles and Its Application in Determination of BSA. [J]. 33(10):1209-1212(2014)
DOI：

Flourescence Enhancement of CT2R by Silver Nanoparticles and Its Application in Determination of BSA. [J]. 33(10):1209-1212(2014) DOI：

摘要

利用酸性条件下，牛血清白蛋白（BSA）可降低银纳米粒子-变色酸2R（CT2R）体系的表面增强荧光效应，建立了一种测定BSA的荧光分析新方法。考察了pH值、CT2R的浓度、银纳米粒子浓度、试剂加入顺序和共存物质等因素对测定BSA的影响。实验结果表明，在pH值为5.72，CT2R的浓度为1.5×10-5 mol/L，银纳米粒子浓度（以银原子计算）为1.25×10-4 mol/L，按银纳米粒子、BSA、CT2R、BR缓冲溶液依次添加的条件下，BSA的线性范围为0.02～1.00 mg/L，检出限为0.002 6 mg/L。该法用于合成样品中BSA的测定，灵敏度高，重现性好，结果准确。

Abstract

Under acidic conditions，the flourescence of CT2R could be enhanced with silver nanoparticles because of its surface enhancement effect.This enhancement effect would be decreased in the presence of BSA.The decreased fluorescence intensity is proportional to the concentration of BSA.According to this principle，a simple and new method for BSA determination was developed.The influences of some experimental factors，including pH value，concentration of CT2R，concentration of silver nanoparticles，addition order of reagents on the determination of BSA were investigated.Under the optimal conditions，the linear range for BSA detection is 0.02-1.00 mg/L，with a detection limit of 0.002 6 mg/L.The method was used in the detection of BSA in synthetic samples with satisfactory results.

关键词

CT2R银纳米粒子荧光光谱表面增强荧光

Keywords

chromotropic acid 2Rsilver nanoparticlesfluorescence spectroscopysurface enhancement effect

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