The electrochemical behavior of Ibuprofen(IB) on the graphene/DNA/gold nanoparticles(Gr/DNA/GNPs) modified electrode was studied. The Gr/DNA/GNPs composites were characterized by UV-visible spectrophotometry and scanning electron microscopy techniques. The detection effects of different modified electrodes were compared,and effects of experimental conditions such as buffer system and modification amount on the determination were discussed. The results showed that,IB had a well defined electrochemical signal on Gr/DNA/GNPs composite modified electrode,and a sensitive oxidation peak could be observed at 0.83 V in 0.1 mol?L-1 PBS buffer solution (pH 6.8). Under the optimal conditions,the linear range of IB was in the range of 7.2×10-7-4.9×10-5mol?L-1,and the detection limit was 1.5×10-7mol?L-1.The interference test and repeated experiments showed that the modified electrode had a good selectivity and reproducibility,and was applied in the detection of IB in real samples with a satisfactory results.