Fluorescence Quenching Reaction of Cefotaxime Sodium with Acriflavine and Their Analytical Applications

您当前的位置：

首页 >

文章列表页 >

Fluorescence Quenching Reaction of Cefotaxime Sodium with Acriflavine and Their Analytical Applications

- Fluorescence Quenching Reaction of Cefotaxime Sodium with Acriflavine and Their Analytical Applications
- Vol. 33, Issue 4, Pages: 460-464(2014)
- 作者机构：
  
  1. 重庆市巴南区环境监测站
  2. 西南大学化学化工学院
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
Fluorescence Quenching Reaction of Cefotaxime Sodium with Acriflavine and Their Analytical Applications. [J]. 33(4):460-464(2014)
DOI：

Fluorescence Quenching Reaction of Cefotaxime Sodium with Acriflavine and Their Analytical Applications. [J]. 33(4):460-464(2014) DOI：

摘要

在pH 2.1~4.1的Britton-Robinson（BR）缓冲溶液中，头孢噻肟钠（CFTM）与吖黄素（AF）形成1∶1的离子缔合物，导致吖黄素溶液荧光猝灭。当分别于最大激发和最大发射波长（λex/λem=266 nm/506 nm）进行测量时，荧光猝灭值（ΔF）与CFTM浓度在一定范围呈良好的线性关系。该方法灵敏度高，测定CFTM的线性范围和检出限分别为0.07~5.0 mg/L 和0.021 mg/L。考察了体系的荧光光谱特征、适宜的反应条件和共存物质的影响，讨论了离子缔合物的组成。基于离子缔合反应，发展了测定CFTM的高灵敏、简便、快速的新方法，将其用于血清和尿液中CFTM的测定，结果满意。

Abstract

In Britton-Robinson（BR） buffer solution at pH 2.1-4.1,acriflavine（AF） reacted with cefotaxime sodium（CFTM） to form an ion association complex that quenched the fluorescence of acriflavine and whose compound ratio was 1∶1.The measurement wavelength of fluorescence quenching was located at λex/λem=266 nm/506 nm.The linear range for CFTM ranged from 0.07 mg/L to 5.0 mg/L,and its detection limit was 0.021 mg/L.In this study,the fluorescence spectra characteristics,the optimum reaction conditions and effect of foreign substances were investigated.The composition of ion-association complexes was discussed.Based on the ion-association reaction,a high sensitive,simple and rapid method for the determination of CFTM was proposed.The method was applied for the determination of CFTM in human serum and urine samples with satisfactory results.

关键词

头孢噻肟钠吖黄素荧光猝灭

Keywords

cefotaxime sodiumacriflavinefluorescence quenching

references

Views

955

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

One-step Hydrothermal Synthesis of Nitrogen-doped Carbon Dots for Fe³⁺ Detection and Their Application in Cellular Imaging

Conformational Changes of Human Serum Albumin and Bovine Serum Albumin Induced by 1-Naphthol

Scientific PapersSynthesis of Nitrogen-doped Carbon Quantum Dots and Their Applications in Cellular Imaging and o-Nitrophenol Detection

Determination of Celastrol by Lanthanide Sensitized Fluorescence Quenching on Terbium- Enoxacin-SDS

A New Method for Determination of Mercury Ions Based on the Fluorescence Quenching of Phycocyanin from Spirulina Platensis

Related Author

No data

Related Institution

Scientific Research Center，Qiqihar Medical University

Research Institute of Medicine and Pharmacy，Qiqihar Medical University

Heilongjiang Industrial Hemp Processing Technology Innovation Center，Qiqihar University

College of Chemistry and Chemical Engineering，Qiqihar University

Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰