A rapid method was established for determination of five fluoroquinolones antibiotic residues in milk.Sample solution was injected after protein precipitating with 0.2% trichloroacetic acid-acetonitrile(90∶10) .Turboflow column was used as SPE column to enrich sample through large volume injection and cleanup with left pump of dual gradient LC.Strong solvent stored in 100 micro-liter loop was used to transfer the analyte which was diluted with right pump of DGLC in online mode.Then peak shape and resolution were improved by peak refocusing effect.The limits of detection(LODs) were between 0.01 μg/L and 0.13 μg/L by fluorescence detection，which were far below the maximum residue levels for milk set by the EU regulations. The linearity of this method was good in the certain concentration range.The mean recoveries were in the range of 75.9%- 95.7%.The results indicated that this method is simple，rapid and sensitive，and is suitable for the determination of five fluoroquinolones antibiotic residues in milk.