Detection of Carcinoembryonic Antigen(CEA) in Human Serum Using Label-free Amperometric Electrochemical Immunosensor

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Detection of Carcinoembryonic Antigen(CEA) in Human Serum Using Label-free Amperometric Electrochemical Immunosensor

- Detection of Carcinoembryonic Antigen(CEA) in Human Serum Using Label-free Amperometric Electrochemical Immunosensor
- Vol. 33, Issue 7, Pages: 786-791(2014)
- 作者机构：
  
  1. 福建医科大学药学院药物分析学系
  2. 福建医科大学附属协和医院骨科
- 作者简介：
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Detection of Carcinoembryonic Antigen(CEA) in Human Serum Using Label-free Amperometric Electrochemical Immunosensor. [J]. 33(7):786-791(2014)
DOI：

Detection of Carcinoembryonic Antigen(CEA) in Human Serum Using Label-free Amperometric Electrochemical Immunosensor. [J]. 33(7):786-791(2014) DOI：

摘要

构建了灵敏的铁氰化钾-壳聚糖-戊二醛信号体系，并以此为信号指示剂，建立了稳定、准确的免标记电化学免疫传感器用于血清中癌胚抗原(CEA)的检测。信号体系和Nafion分别修饰于玻碳电极表面，并固定CEA抗体，分别用原子力显微镜(AFM)和循环伏安法(CV)对电极修饰过程的形貌和电化学行为进行表征。结果表明，循环伏安的电流响应值与固定在电极表面的CEA浓度直接相关，且CEA浓度的对数值在0.005~40.0 ng/mL范围内与电流的降低值呈良好的线性关系，检出限为 1.23 pg/mL。方法具有良好的特异性，能准确检测血清样本中CEA的浓度。

Abstract

A label-free immunosensor based on K3［Fe(CN)6］-chitosan-glutaraldehyde conjugate composite as signal indicator was developed for the easy and sensitive detection of carcinoembryonic antigen(CEA).The signal layer and nafion-modified glass carbon electrode(GCE) was used to wrap CEA antibodies.The morphology and electrochemical behaviors of the modified GCE substrates were characterized by atomic force microscopy(AFM) and cyclic voltammetry(CV),respectively.The result indicated that the response magnitudes of the anodic peak current were directly related to the concentration of CEA.A good linear relationship between decrease of current and logarithm of CEA concentration in the range of 0.005-40.0 ng/mL was obtained,with a detection limit of 1.23 pg/mL.The developed approach showed a high selectivity,and could be applied in the detection of CEA in human serum samples.

关键词

癌胚抗原铁氰化钾-壳聚糖-戊二醛信号体系免标记免疫传感器循环伏安法

Keywords

carcinoembryonic antigenK3［Fe(CN)6］-chitosan-glutaraldehyde conjugatelabel-free immunosensorcyclic voltammetry

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Guangxi Liuzhou Luosifen Research Center of Engineering Technology， Guangxi Key Laboratory of Green Processing of Sugar Resources， College of Biological and Chemical Engineering， Guangxi University of Science and Technology

Liuzhou Institutes for Food and Drug Control

Province and Ministry Co-sponsored Collaborative Innovation Center of Sugarcane and Sugar Industry

河南工业大学化学化工学院

巢湖学院化学与生命科学学院

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