A liquid chromatography coupled with tandem mass spectrometric(LC-MS/MS) method was developed for the simultaneous determination of streptomycin and dihydrostreptomycin residues in eels and honey samples.The samples were extracted with sodium 1-heptanesulfonate and phosphate buffer,and cleaned up with a SUPELCO LC-C18 solid phase extraction(SPE) column.The extracts were separated on a Thermo Aquasil-C18 column(150 mm×4.6 mm,3 μm) using acetonitrile containing 0.3% acetic acid -0.3% acetic acid as mobile phase by gradient elution.The detection of streptomycin and dihydrostreptomycin was carried out by MS/MS under multiple reaction monitoring(MRM) mode.An external standard method was used for quantitative analysis.The method showed good linearities in the range of 10-100 μg/kg for streptomycin and dihydrostreptomycin,and the limits of quantitation(LOQ) were 10 μg/kg.The spiked recoveries of streptomycin and dihydrostreptomycin ranged from 89.6% to 110.3% with relative standard deviations of 2.3%-20.9%.The method was simple,rapid and practical,and could meet the requirements for determination of streptomycin and dihydrostreptomycin residues in eel and honey samples.