Determination of Quercetin in Herbal Gynostemma Pentaphyllum by Using Excitation-Emission Matrix Fluorescence Coupled with Second-order Calibration Method

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Determination of Quercetin in Herbal Gynostemma Pentaphyllum by Using Excitation-Emission Matrix Fluorescence Coupled with Second-order Calibration Method

- Determination of Quercetin in Herbal Gynostemma Pentaphyllum by Using Excitation-Emission Matrix Fluorescence Coupled with Second-order Calibration Method
- Vol. 33, Issue 9, Pages: 1038-1043(2014)
- 作者机构：
  
  河北师范大学化学与材料科学学院
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Determination of Quercetin in Herbal Gynostemma Pentaphyllum by Using Excitation-Emission Matrix Fluorescence Coupled with Second-order Calibration Method. [J]. 33(9):1038-1043(2014)
DOI：

Determination of Quercetin in Herbal Gynostemma Pentaphyllum by Using Excitation-Emission Matrix Fluorescence Coupled with Second-order Calibration Method. [J]. 33(9):1038-1043(2014) DOI：

摘要

采用三维荧光并结合二阶校正的平行因子分析(PARAFAC)算法、交替三线性(ATLD)算法和自加权交替三线性(SWATLD)算法对中药绞股蓝中的槲皮素含量和回收率进行测定，Al(Ⅲ)作敏化剂增加槲皮素的荧光强度。核一致检验法说明体系中荧光组分数为2，其中一个组分对应于槲皮素，另一个对应于中药中的一个共存组分。3种方法测定中药绞股蓝中槲皮素的含量分别为0.274 9%，0.272 9%和0.272 7%，回收率分别为96.3%，103.1%和96.8%，得到的槲皮素解析光谱和真实光谱几乎完全重合。实验结果表明，化学计量学“二阶校正”法具有简单、快速、高效及操作费用低等特点，可用于复杂成分的定性和定量分析。同时，还讨论了中药溶液中内滤光效应对荧光强度及定量分析的影响，并采用数学校正法对中药溶液的内滤光效应进行校正，实验发现测定的荧光强度用相应的溶液吸光度校正后，定量分析结果的准确度大大改善。

Abstract

A simple，rapid and effective method for the quantitative analysis of quercetin in Gynostemma pentaphyllum was established by using excitation-emission matrix (EEM) fluorescence coupled with second-order calibration based on Parallel factor analysis(PARAFAC) algorithm，alternating trilinear decomposition (ATLD) algorithm and self weighted alternating trilinear decomposition (SWATLD) algorithm.Al(Ⅲ) was used as fluorescence sensitizer to increase the quercetin fluorescence intensity.The core-consistency diagnostic(CORCONDIA) test indicated the number of components should be two，one of the components corresponds to the quercetin，and the other is related to a coexistence component of traditional Chinese medicine.The content of quercetin by PARAFAC，ATLD，SWATLD with component number N=2 are 0.274 9%,0.272 9% and 0.272 7%，and the average recoveries were 96.3%，103.1% and 96.8%，respectively.The resolved quercetin spectra overlap almost completely with real spectra.The experimental results showed that the chemometrics “second order correction” method was simple，rapid，high efficient and low cost，and could be used for complex component qualitative and quantitative analysis.In addition，the influence of the inner filter effect on fluorescence intensity was discussed，and the results revealed that ultraviolet correction could effectively improve the accuracy of quantitative analysis.

关键词

二阶校正法三维荧光内滤光效应吸光度校正槲皮素

Keywords

second-order calibration3D fluorescenceinner filter effectsabsorbance correctionquercetin

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