Study on a Phorate Biosensor Design Based on Acetylcholinesterase Immobilized with Functionalized Graphene-Polyvinyl Alcohol Nanocomposite

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Study on a Phorate Biosensor Design Based on Acetylcholinesterase Immobilized with Functionalized Graphene-Polyvinyl Alcohol Nanocomposite

- Study on a Phorate Biosensor Design Based on Acetylcholinesterase Immobilized with Functionalized Graphene-Polyvinyl Alcohol Nanocomposite
- Vol. 34, Issue 10, Pages: 1152-1157(2015)
- 作者机构：
  
  河南工业大学化学化工学院
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Study on a Phorate Biosensor Design Based on Acetylcholinesterase Immobilized with Functionalized Graphene-Polyvinyl Alcohol Nanocomposite. [J]. 34(10):1152-1157(2015)
DOI：

Study on a Phorate Biosensor Design Based on Acetylcholinesterase Immobilized with Functionalized Graphene-Polyvinyl Alcohol Nanocomposite. [J]. 34(10):1152-1157(2015) DOI：

摘要

将功能化离子液体修饰石墨烯(IL-GR)分散在聚乙烯醇（PVA）中，制得IL-GR-PVA分散液，与乙酰胆碱酯酶(AChE)溶液混匀后滴涂在电极表面，利用PVA良好的成膜特性，制得新型有机磷检测酶电极AChE/IL-GR-PVA/GCE,并用于有机磷农药的检测。采用透射电镜（TEM）表征了IL-GR的形貌，采用循环伏安法(CV)和差示脉冲伏安法(DPV)研究了酶电极的电化学性质。结果表明，IL-GR-PVA复合膜具有良好的导电性和生物相容性，能很好地保持AChE的生物活性,并显著促进了其电化学过程。在优化实验条件下，抑制率(I%)与甲拌磷浓度的负对数在1.0×10-14 ~1.0×10-9 mol/L和1.0×10-9~1.0×10-6 mol/L范围内呈良好的线性关系，检出限(S/N=3)为8.0×10-15 mol/L。该传感器制备简单，稳定性好，灵敏度高，为有机磷农药的测定提供了新方法。

Abstract

Ionic liquid modified graphene(IL-GR) was firstly dispersed in polyvinyl alcohol(PVA) to form IL-GR-PVA dispersion,then IL-GR-PVA and acetylcholinesterase(AChE) was mixed evenly and was coated on the glass carbon electrode via PVA film-forming.A novel biosensor was developed for the detection of organophosphorus pesticide(OP).Different techniques were employed to study the construction process and the electrochemical properties of the biosensor.The IL-GR-PVA nanocomposites with excellent conductivity and biocompatibility offered an extremely hydrophilic surface for AChE adhesion.Linear relationship between the inhibition percentage(I%) and logarithm of phorate concentration was found in the ranges of 1.0×10-14-1.0×10-9 mol/L and 1.0×10-9-1.0×10-6 mol/L,with a detection limit(S/N=3) of 8.0×10-15 mol/L.The easily prepared electrochemical sensor has a favorable stability and sensitivity,thus providing a new OPs detection method

关键词

石墨烯离子液体聚乙烯醇甲拌磷传感器

Keywords

grapheneionic liquidpolyvinyl alcoholphoratebiosensor

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Related Institution

School of Chemical Engineering，Guizhou University for Nationalities

Institute of Tea Research，Guizhou Academy of Agricultural Sciences

Laboratory of Chemistry of Xinjiang Specialty Medicinal and Edible Plant Resources， Kashgar University，Kashgar

沈阳农业大学植物保护学院

广东第二师范学院化学系

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