An extraction method of heliotrine was developed by focused microwave-assisted extraction(FMAE).Orthogonal design was used to optimize the experimental conditions.The optimum extraction conditions were as follows: 60 mesh size of material，solvent-materail ratio:1∶35，microwave power:420 W and extraction time:50 min.The analyte was determined by HPLC using methanol -0.05%triethylamine(pH 7.5 )(30∶70) as mobile phase with a detection wavelength of 220 nm.The linear range of heliotrine was in the range of 52-1 040 mg/L(r=0.999 9)，with a detection limit of 0.040 2 mg/L.The recoveries for heliotrine were in the range of 97.5%-103%with RSDs of 2.4%.The method was simple and highly sensitive.