Determination of Bisphenol A-type Compounds in Canned Foods by High Performance Liquid Chromatography with Fluorescence Detection
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Determination of Bisphenol A-type Compounds in Canned Foods by High Performance Liquid Chromatography with Fluorescence Detection
Vol. 33, Issue 12, Pages: 1393-1398(2014)
作者机构:
1. 上海出入境检验检疫局
2. 欧普图斯光学纳米科技有限公司
3. 上海海洋大学 食品学院
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Determination of Bisphenol A-type Compounds in Canned Foods by High Performance Liquid Chromatography with Fluorescence Detection. [J]. 33(12):1393-1398(2014)
DOI:
Determination of Bisphenol A-type Compounds in Canned Foods by High Performance Liquid Chromatography with Fluorescence Detection. [J]. 33(12):1393-1398(2014)DOI:
Determination of Bisphenol A-type Compounds in Canned Foods by High Performance Liquid Chromatography with Fluorescence Detection
A method for the determination of 7 bisphenol A-type compounds in canned foods by high performance liquid chromatography with fluorescence detection(HPLC/FLD) was established.Samples were extracted with acetonitrile,and cleaned up with a polyvinylpyrrolidone(PLS) cartridge.Chromatographic separation was carried out on a phenyl column(250 mm×4.6 mm,5 μm) with acetonitrile-water as mobile phase by gradient elution.The baseline separation of seven bisphenol A-type compounds was obtained.Excitation and emission wavelengths of fluorescence detection were set at 227 nm and 313 nm,respectively.For seven bisphenol A-type compounds,there were good linear relationships between concentrations and peak areas in the range of 0.010-5.0 μg/mL,and the correlation coefficients(r2) were greater than 0.99.The average recoveries of 7 bisphenol A-type compounds at fortified levels of 0.015-5.0 mg/kg ranged from 83.7% to 95.5%,with relative standard deviations(RSDs) of 7.2%-19.8%.The limits of detection(LOD) and quantitation(LOQ) were in the range of 5-18 μg/kg and 15-50 μg/kg,respectively.This method was accurate,sensitive and reliable,and could be applied in the real sample analysis.
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