Sulfaguanidine can significantly inhibit the chemiluminescence(CL) intensity of Ni(Ⅳ) complex-luminol system.Based on this，a new method of Ni(Ⅳ) complex-luminol CL system coupled with flow-injection for the determination of sulfaguanidine is developed.Under the optimum conditions，the linear range is 6.0×10-7 mol/L to 6.0×10-6 mol/L to the CL relative intensities with a detection limit(3σ) of 2.4×10-10 mol/L.The RSD(n=11) is 1.4%for 2.4×10-6 mol/L sulfaguanidine.The proposed method was applied in the analysis of sulfaguanidine in swine feed samples with satisfactory results.