In this paper,hordein nanoparticles were self assembled by liquid–liquid dispersion method.Effects of different parameters,including hordein concentration,isopropanol concentration,dispersion solution pH value and ionic strength on particle size and Zeta potential of self-assembled nanoparticles were systematically investigated.Smaller nanoparticles (70.0±1.8 nm) with Zeta potential 9 mV can be formed under the conditions of 55% isopropanol concentration,66 mg/mL hordein, pH 7.0 dispersion solution,0 ionic strength.Moreover,resveratrol was successfully encapsulated into hordein nanoparticles.The effects of resveratrol/hordein weight ratio on particle size,encapsulation efficiency and drug loading of resveratrol-hordein nanoparticles were studied.The results showed that the composite nanoparticles with (135.3±2.5) nm diameter,(18.91±0.02) mV Zeta potentials,90.4%encapsulation efficiency,and 18.8% drug loading were obtained under the condition of resveratrol/hordein weight ratio 1∶5.The morphological structures of both nanoparticles were smooth and spherical.The fourier transform infrared spectroscopy results showed that the electrostatic interactions and hydrogen bonds exist between resveratrol and hordein.