A comprehensive analytical method based on ultra performance liquid chromatography tandem mass spectrometry was developed for the simultaneous determination of fifteen prohibited androgens(e.g.adrenosterone,trenbolone,boldenone,fluoxymesterone,oxandrolone,androstenedione,nandrolone,metandienone,testosterone,dehydroepiandrosterone,clostebol,stanolone,mestanolone,4-chlorotestosterone acetate and testosterone propionate).Various cosmetic samples were extracted with methanol by ultrasonication.The extract was centrifuged,and the upper solution was then cleaned up with an Oasis HLB solid phase extraction cartridge.After chromatographic separation on an ACQUITY UPLC BEH C18(2.1 mm×50 mm,1.7 μm) column,the target compounds were analyzed under the multiple reaction monitoring(MRM) mode in positive ionization mode.The limits of quantitation(LOQs) for fifteen androgens were in the range of 1.0-20.0 μg/kg.The mean recoveries at three spiked levels ranged from 76.5% to 92.1%,with relative standard deviations of 1.3%-9.6%.The method was proved to be accurate,rapid and sensitive,and could provide a scientific basis and technical support for the inspection and production quality control of anti-acne cosmetics.