A method for the simultaneous separation of seven kinds of drugs including tetracyclines（TCs） and penicillins（PENs） by micelle capillary electrophoresis（MEKC） was developed.Effects of some important factors such as composition，pH value and concentration of running buffer，surfactant concentration，separation voltage and separation temperature were investigated，and the factors were optimized by the L16（45） orthogonal test.The optimal conditions were as follows：the running buffer：40 mmol/L potassium dihydrogen phosphate-20 mmol/L borax solution（pH 7.9），SDS concentration：65 mmol/L,separation voltage：28 kV，separation temperature：28 ℃，UV detection wavelength：350 nm and 200 nm．Seven kinds of drugs were baseline separated within 25 min.The calibration curves showed good linearities in the range of 1.56-50 mg/L，with correlation coefficients（r2） of 0.997 9-0.999 9．RSDs（n=6） were in the range of 4.1%-7.3% for peak area and 0.33%-0.67% for migration time.The average recoveries of seven kinds drugs at three spiked concentration levels of 2.0，5.0，10.0 mg/kg ranged from 83.6% to 93.3%，with RSDs（n=6） of 4.7%-7.6%．The method was rapid，simple，accurate and high sensitive,and was successfully applied in the determination of seven kinds of drugs in the ponds and lakes of Hefei and its surrounding areas.