A method has been developed for simultaneous determination of triptolide,triptonide and triptophenolide in traditional Chinese herb Tripterygium wilfordii Hook F.by micellar electrokinetic capillary chromatography（MEKC） combined with activated carbon solid phase extraction.Effects of some important factors,such as pH value of extraction,eluant volume of ethanol，pH value and concentration of running buffer,concentration of sodium dodecyl sulfate（SDS），separation voltage and injection time were investigated.The analytes were extracted with activated carbon in phosphate buffer solution（pH 6.5）,then eluted with 2.0 mL ethanol before separated by MEKC.At 214 nm wavelength,the detection analytes could be well separated within 8 min at separation voltage of 20 kV in 20 mmol/L boric acid-10 mmol/L borax（pH 8.0）-20 mmol/L SDS.Under the optimized conditions,the calibration curves were linear in the range of 4.0×10-5-4.0×10-3 mol/L for triptolide and the triptonide,and 4.0×10-5-1.0×10-3 mol/L for triptophenolide.The detection limits of triptolide,triptonide and triptophenolide were 1.42×10-6,7.90×10-7,2.96×10-7 mol/L,respectively,and the relative standard deviations（RSDs,n=6） for peak areas were 3.2%,5.4%,3.5%,respectively.The proposed method had the advatages of simplicity,rapidness,low sample consumption and accuracy,and was successfully applied in the determination of the analytes in Tripterygium wilfordii tablet with recoveroes of 81.0%-102.9%.