Amplified Optical Aptasensor through  Exonuclease Ⅲ Stimulated Regeneration of  ATP Based on Graphene

您当前的位置：

首页 >

文章列表页 >

Amplified Optical Aptasensor through Exonuclease Ⅲ Stimulated Regeneration of ATP Based on Graphene

- Amplified Optical Aptasensor through Exonuclease Ⅲ Stimulated Regeneration of ATP Based on Graphene
- Vol. 34, Issue 10, Pages: 1168-1172(2015)
- 作者机构：
  
  广西师范大学化学与药学学院药用资源化学与药物分子工程教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
Amplified Optical Aptasensor through Exonuclease Ⅲ Stimulated Regeneration of ATP Based on Graphene. [J]. 34(10):1168-1172(2015)
DOI：

Amplified Optical Aptasensor through Exonuclease Ⅲ Stimulated Regeneration of ATP Based on Graphene. [J]. 34(10):1168-1172(2015) DOI：

摘要

基于核酸外切酶Ⅲ(Exo Ⅲ)辅助分析物循环放大原理，结合石墨烯(G)的超高荧光猝灭能力，以三磷酸腺苷(ATP)作为模型分析物，构建了一种新型的荧光探针，实现了ATP的高灵敏检测。两条DNA链各包含一部分ATP适体序列，其中1条DNA链的3 端修饰有荧光染料异硫氰酸荧光素(FITC)。当模型分析物不存在时，两条DNA链通过非共价π-π堆积作用吸附在G表面，导致FITC的荧光猝灭。当目标物ATP存在时，两条DNA链与目标物ATP进行特异性结合，形成一双链夹心结构，随后加入Exo Ⅲ，由于Exo Ⅲ可对该双链夹心结构的3凹陷末端进行逐步水解，释放出目标物ATP和游离的FITC。释放出的目标物ATP可继续进入下一循环，不断产生游离FITC，游离FITC将脱离G表面，从而导致体系的荧光恢复，并且恢复的荧光强度与ATP浓度在0.02~1 μmol/L范围内存在良好的线性关系，检出限(S/N=3)为9 nmol/L。

Abstract

A highly sensitive platform for adenosine triphosphate(ATP)assay was designed based on exonuclease Ⅲ(Exo Ⅲ)induced target recycling amplification and fluorescence quenching of graphene.The system mainly consists of two nucleic acid strands P1 and P2 carrying a part of aptamer sequences for ATP,and P1 with FITC labeled on its 3’ end.Upon addition of target ATP to the system,P1 hybridizes with P2,and forms a DNA duplex sandwich structure.The formation of DNA duplex sandwich structure triggers selective enzymatic cleavage of 3-recessed end by Exo Ⅲ,resulting in the release of target ATP and FITC.Released target ATP then hybridizes with nucleic acid strands P1 and P2,generating many FITC molecule.As a result,the FITC molecule break away from the G surface,and lead to the fluorescence recovery of the dye FITC.This assay exhibits a high sensitivity with a detection range of 0.02-1 μmol/L and a detection limit(S/N=3) of 9 nmol/L.

关键词

三磷酸腺苷核酸外切酶Ⅲ石墨烯荧光法适体传感器

Keywords

adenosine triphosphateexonuclease Ⅲgraphenefluorometryaptasensor

references

Views

1433

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Aptamer-Based Electrochemical Biosensing

Detection of 4-Iodophenoxyacetic Acid Using a Graphene-loaded Tin Dioxide Electrochemical Sensor

Progress in DNA Sequencing Based on Graphene Nanomaterials

In-situ Preparation of a Solid Phase Microextraction Probe Based on Graphene Self assembly and Its Application in Determination of Benzene Series in Water

Application of Smartphone based Detection in Food Safety

Related Author

No data

Related Institution

School of Chemical Engineering and Technology，Sun Yat-sen University

Center for Advanced Analytical Science & Guangzhou Key Laboratory of Sensing Materials and Devices & Guangdong Engineering Technology Research Center for Sensing Materials and Devices，School of Chemistry and Chemical Engineering，Guangzhou University

Laboratory of Chemistry of Xinjiang Specialty Medicinal and Edible Plant Resources， Kashgar University，Kashgar

Institute of Tea Research，Guizhou Academy of Agricultural Sciences

School of Chemical Engineering，Guizhou University for Nationalities

Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰