A method was developed for the determination of benzo［α］ pyrene in Sarcandra galbra by solid phase extraction/high performance liquid chromatography（HPLC） with fluorescence detector.The benzo［α］ pyrene in Sarcandra galbra was extracted with n-hexane,and purified with neutral alumina column.The prepared samples were analyzed by HPLC with fluorescence detector at λex=370 nm and λem=425 nm,using acetonitrile-water（85∶15） as mobile phase at a flow rate of 1.0 mL/min.The results showed that a good linearity was achieved in the range of 0-20 ng/mL for benzo［α］ pyrene with a correlation coefficient of 0.999 9.The limit of quantitation（LOQ） was 0.36 μg/kg.The mean recoveries of benzo［α］ pyrene were in the range of 84.4%-101.9% with relative standard deviations（RSDs） of 1.6%-5.6%.The method is accurate,sensitive and repeatable,and is suitable for the analysis of benzo［α］ pyrene in traditional Chinese herbal medicines.