In order to establish a simple and rapid method for the determination of sophocarpine,the gold electrode modified with silica sol/Nano-Au/PVP was prepared,which could be used to detect sophoridine by ECL based on the obvious electrochemiluminescence sensitization effect of sophoridine on Ru(bpy)2+3.Under the optimum conditions,the ECL intensities presented a good linearity with concentration of sophoridine in the range of 1.6×10-7-1.6×10-4 mol/L,with a linear equation of I=4.000×107c+262.2(r2=0.994 5) and a detection limit(S/N=3) of 3.5×10-9 mol/L.RSD(n=6) for determination of 1.6×10-5 mol/L sophoridine was 2.9%.The proposed method showed a high selectivity,sensitivity and simplicity,and could be applied in the analysis of sophoridine with satisfactory results.