The purified performance of three different types of solid-phase extraction columns,including HLB column,C18 column and PEP column,was investigated in this study.And based on that,a solid phase extraction/high performance liquid chromatography was developed for the simultaneous determination of 8 antibiotic residues in dairy.The result indicated that the HLB column showed an excellent elution and purification effect,and it was applicable for the dairy matrix purification.Under the optimal conditions,the calibration curves of eight antibiotics were linear in the concentration range of 5.0-100.0 μg/L with correlation coefficients(r2) more than 0.999.The limits of detection(LOD) and limits of quantitation(LOQ) of 8 antibiotics were in the range of 0.09-0.84 μg/kg and 0.32-2.23 μg/kg, respectively.The recoveries of target compounds at spiked concentration levels of 5,10 and 30 μg/kg ranged from 73.2% to 93.3%,with relative standard deviations(n=5) of 1.4%-3.6%.The developed method was suitable for the analysis of antibiotic residues in the complex dairy matrix.