A confirmatory method was developed to determine 5 alkaloids including morphine,codeine,thebaine,papaverine and noscapine by high performance liquid chromatography quadrupole-orbitrap high resolution mass spectrometry（HPLC-Q/Exactive） in foodstuffs,such as chafing dish materials,condiments,barbecue,and liangpi.The sample was extracted with hydrochloric acid solution in ultrasonic assisted condition,and chloroform was used for removing the fat.The purification was carried out with an ion exchange solid phase extraction（SPE） column,using the ammoniated methanol-ethyl acetate as eluent.The separation of analytes was performed on an Accucore aQ column.To finish the accurate screening poppy features ingredients in food,the molecular ion accurate mass was used by orbitrap full scan in electro-spray ionization,while product ions full scan were triggered by automatically setting the threshold.The quantification was implemented with the isotope internal standard.Meanwhile,the possible fragmentation patterns were studied.Under the optimized experimental conditions,the obtained mass accuracy errors of 5 analytes were less than 5 ppm.The limit of quantitation（LOQ） was 2.0 μg/kg for morphine,0.2 μg/kg for codeine,0.1 μg/kg for papaverine,thebaine and noscapine.Good linear relationships（r2> 0.999） were obtained between analyte concentrations and peak areas.The method recoveries were in the range of 63.4%-112.8%,and the relative standard deviation were 5.5%-13.6%.This method was used to analyze actual foodstuffs.The detected results showed that the quantification data was accurate and the confirmation data was reliable.