A rapid method was developed for the simultaneous determination of 20 synthetic colors in cured meat products by high performance liquid chromatography(HPLC) with sub-3 μm columns.The samples were degreased with petroleum benzin，extracted with ethanol-ammonium hydroxide-water(7∶2∶1) and deproteinized with sodium tungstate solution.The target compounds were separated on an Agilent Poroshell 120 EC C18 column(50 mm×4.6 mm,2.7 μm) using methanol-0.02 mol/L ammonium acetate solution(pH 5.5) as mobile phase with gradient elution，and detected with DAD.The quantification analysis of analytes was performed by the external standard method.The result indicated -that 20 analytes were separated completely in 17 min，and their calibration curves were linear in the range of 0.15-20.0 mg/L with correlation coefficients more than 0.998. The average recoveries at three spiked levels ranged from 70.3% to 107.9% with RSDs(n=6) of 0.5%-3.1%.The detection limits were in the range of 0.1-0.2 mg/kg. The method approximately reduced assay time by 62% compared with that of the regular ACE C18 column(250 mm×4.6 mm，5 μm).The method could be performed routinely on a conventional LC system,which indicates an extensive application prospect.