In this paper,SiO2-coated carbon nanotubes（CNTs/SiO2） were synthesized by sol-gel technique.The vinyl groups were introduced onto the surface of CNTs/SiO2 through chemical modification with γ-methacryloxypropyl trimethoxysilane（MAPS）.Through free radical polymerization,CNTs/SiO2-MIPs were successfully obtained by the copolymerization in presence of MAPS-CNTs/SiO2 as support,rutin as template molecule,acrylamide as functional monomer and ethylene glycol dimethacrylate as crosslinker.CNTs/SiO2-MIPs were characterized by infrared spectrometry,thermal gravimetric analysis and scanning electronic microscopy.CNTs/SiO2-MIPs were coated onto glassy carbon electrode to construct an electrochemical sensor.The electrochemical characteristics of CNTs/SiO2-MIPs modified electrode were investigated by cyclic voltammetry and differential pulse voltammetry.The results showed that CNTs/SiO2-MIPs had a good specific adsorption property.DPV peak current response of CNTs/SiO2-MIPs sensor was nearly 2.84 times that of the non-imprinted polymers.In addition,the CNTs/SiO2-MIPs sensor could recognize rutin from its relatively similar molecules of quercetin,naringenin and ascorbic acid.The CNTs/SiO2-MIPs sensor had a wide linear range over rutin concentration range of 0.1-100.0 μmol/L with a detection limit（S/N=3） of 0.032 μmol/L.The CNTs/SiO2-MIPs imprinted sensor was successfully used for the determination of rutin in tablets.